recombinant human cxcl16  (R&D Systems)

Journal: Clinical Cancer Research

Article Title: Redirecting B7-H3.CAR T Cells to Chemokines Expressed in Osteosarcoma Enhances Homing and Antitumor Activity in Preclinical Models

doi: 10.1158/1078-0432.CCR-23-3298

Figure Lengend Snippet: RNA-seq and multiplex analyses identify chemokines produced by primary OS samples. A, Expression of 28 chemokines by RNA-seq (FPKM shown; n = 85 OS samples); screen cutoff shown at 1 FPKM. B, Expression of 28 chemokines by protein secretion ( n = 8 patient samples); screen cutoff shown at 3 × 10 3 integrated pixel density. Green bars in A and B denote ligands deemed positive in both RNA and protein screens; error bars indicate SEM. C, Chemokine receptors (ligands in parentheses) CXCR2 (IL8), CXCR3 (CXCL10), CXCR6 (CXCL16), CCR7 (CCL21), and CCR8 (CCL18) as expressed endogenously and detected by flow cytometry on activated T cells ( n = 5 healthy donors; error bars indicate SEM). D, ELISA demonstrating detection of IL8 and CXCL16 in patient transport media specimens ( n = 6 patient tumor samples, 1 normal bone sample; error bars indicate SEM).

Article Snippet: At time point 0, the transwell was placed into a fresh V-shaped lower-chamber plate with 100 μL of RPMI supplemented with 1% FBS and 0, 10, or 30 ng/mL of recombinant human IL8 (R&D Systems) or recombinant human CXCL16 (R&D Systems).

Techniques: RNA Sequencing Assay, Multiplex Assay, Produced, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: Distinct molecular and immune hallmarks of inflammatory arthritis induced by immune checkpoint inhibitors for cancer therapy

doi: 10.1038/s41467-022-29539-3

Figure Lengend Snippet: a UMAP view of the distribution of top 100 expanded T cell clones in natural killer (NK), NK T, and T cell clusters (left), number of top 100 expanded T cell clones across clusters from each patient (middle), and annotation of the 17 cell clusters (right). n = 8 PB and matching SF samples. PB, peripheral blood; SF, synovial fluid; TCR, T cell receptor; Treg, regulatory T cells; MAIT, mucosal-associated invariant T cells. b Heatmap showing T cell repertoire overlap across clusters. Numbers indicate the number of shared clonotypes between each cluster pair. Statistically significant overlap of T cell clones is indicated by colored boxes, based on a one-sided Fisher’s Exact test followed by adjusting the false discovery rate using the Benjamini-Hochberg method. n = 8 PB and matching SF samples. c Circos plots showing interactions between major immune cells subsets via chemokine receptors (CXCR3 and CXCR6) and their ligands (CXCL9/10/11 and CXCL16). The lines were colored based on their statistically significance and the weight of the lines denotes their corresponding gene expression levels, the higher the expression level, the thicker the line. n = 8 PB and matching SF samples. d Ratio of migrated cells in response to CXCL9/10/11/16 to migrated cells in the absence of the chemokines. n = 6 PB samples from arthritis-irAE patients. Tn: naïve CD8 + T cells; CX3CR1 hi , CX3CR1 hi effector CD8 + T cells. Two-sided paired t test. CX3CR1 hi , ** P = 0.0014. Source data are provided as a Source Data file.

Article Snippet: In the lower chamber, 500 μL of migration medium was placed in the presence/absence of 1 μg/mL of CXCL9 (R&D Systems, Cat #: 392-MG), CXCL10 (R&D Systems, Cat #: 266-IP), CXCL11 (R&D Systems, Cat #: 672-IT), and CXCL16 (R&D Systems, Cat #: 976-CX).

Techniques: Clone Assay, Gene Expression, Expressing